Staff Scientist – Using Single-Cell Genomics to Decode Brain Function
BIDMC and Harvard Medical School
July 25, 2018
Dependent upon skill level and
Full Time - Experienced
Academic / Research
We are seeking scientists interested in using single-cell RNA sequencing to decode brain regions involved in regulating hunger, other homeostatic motivational drives, and physiology. The goals are to: a) create neural “parts lists” for these regions, and then working with a team of neuroscientists, b) develop hypotheses about how these neural “parts” work within circuits to control behavior and physiology, and c) use cell-specific neuroscience technologies to interrogate hypotheses.
Ideal candidates will have experience with RNA-seq, facility with statistics and analyses of big data sets, and be interested in neural circuits controlling homeostatic motivational drives and physiology. Basic programming experience, though not strictly required, is preferred. Candidates must have MS or PhD degrees.
Enabling the above-mentioned goals, our group has state-of-the-art expertise in single neuron and single nuclei transcriptomics (Drop-seq, sNuc-seq, etc.), bioinformatic analysis of single-cell datasets to determine a “parts list” for each brain site (see Campbell JN, et al., Nat Neurosci, 2017), rapid CRISPR/Cas9-mediated generation of recombinase driver mice to provide cell-specific experimental access to newly discovered “parts” (i.e. neurons), and finally cell-specific neuroscience techniques including approaches used for: 1) mapping neural circuitry in mice, 2) manipulating neural activity, in vivo, using optical and/or chemogenetic approaches, 3) monitoring of neural activity in vivo with Ca2+-based imaging and/or optetrode technologies, 4) brain slice electrophysiology, and 5) advanced viral technologies.
Laboratory: Bradford B. Lowell MD, PhD, Professor of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts. Lab website: https://www.lowelllab.com
Recent publications from our collaborative research group:
Campbell JN, Macosko EZ, Fenselau H, Pers TH, Lyubetskaya A, Tenen D, Goldman M, Verstegen AM, Resch JM, McCarroll SA, Rosen ED**, Lowell BB**, Tsai LT**. A molecular census of arcuate hypothalamus and median eminence cell types. Nat Neurosci 20: 484-496, 2017.
Livneh Y, Ramesh RN, Burgess CR, Levandowski KM, Madara JC, Fenselau H, Goldy GJ, Diaz VE, Jikomes N, Resch JM, Lowell BB**, Andermann ML**. Homeostatic circuits selectively gate food cue responses in insular cortex. Nature 546: 611-616, 2017
Fenselau H, Campbell JN, Verstegen AMJ, Madara JC, Xu J, Shah BP, Resch JM, Yang Z, Mandelblat-Cerf Y, Livneh Y and Lowell BB. A rapidly-acting glutamatergic ARCàPVH satiety circuit postsynaptically regulated by a-MSH. Nat Neurosci 20: 42-51, 2017.
Resch JM, Fenselau H, Madara JC*, Wu C, Campbell JN, Lyubetskaya A, Dawes BA, Tsai LT, Li MM, Livneh Y, Ke Q, Kang PM, Fejes-Tóth G, Náray-Fejes-Tóth A, Geerling JC**, Lowell BB**. Aldosterone-sensing neurons in the NTS exhibit state-dependent pacemaker activity and drive sodium appetite via synergy with angiotensin II signaling. Neuron 96, 190–206, 2017.
Mandelblat-Cerf Y, Kim A, Subramanian S, Burgess CR, Tannous BA, Lowell BB**, Andermann ML**. Bidirectional anticipation of future osmotic challenges by vasopressin neurons. Neuron 93: 57-65, 2017
Garfield AS**, Shah BP, Burgess CR, Li MM, Li C, Steger JS, Madara JC, Campbell JN, Kroeger D, Scammell TE, Tannous BA, Myers Jr MG, Andermann**, Krashes MJ**, Lowell BB**. Dynamic GABAergic afferent modulation of AgRP neurons. Nat Neurosci 19: 1628-1635, 2016.
Kong D, Dagon Y, Campbell JN, Guo Y, Yang Z, Yi X, Aryal P, Wellenstein K, Kahn BB**, Sabatini BL**, Lowell BB**. A postsynaptic AMPKàp21-activated kinase pathway drives fasting-induced synaptic plasticity in AgRP neurons. Neuron 91: 25-33, 2016.
Garfield AS, Li C, Madara JC, Shah BP, Webber E, Steger JS, Campbell JN, Gavrilova O, Lee CE, Olson DP, Elmquist JK, Tannous BA, Krashes MJ**, Lowell BB**. A neural basis for melanocortin-4 receptor regulated appetite. Nat Neurosci 18: 863-71, 2015.
Mandelblat-Cerf Y, Ramesh RN, Burgess CR, Patella P, Yang Z, Lowell BB, Andermann ML. AgRP and putative POMC neurons show opposite changes in spiking across multiple timescales. eLife. 2015;4.
Krashes MJ, Shah BP, Madara JC, Olson DP, Strochlic DE, Garfield AS, Vong L, Pei H, Watabe-Uchida M, Uchida N, Liberles SD, Lowell BB. An excitatory paraventricular nucleus to AgRP neuron circuit that drives hunger. Nature 507: 238-42, 2014.
Letters of application and CVs should be emailed to:
Bradford B. Lowell, MD, PhD - email@example.com
Professor of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School
Harvard Medical School and Beth Israel Deaconess Medical Center are Equal Opportunity/Affirmative Action Employers. Women and minorities are particularly encouraged to apply.