Postdoctoral Associate, Yale University: microglial role in early life stress
July 14, 2018
New Haven, Connecticut
Full Time - Experienced
Academic / Research
Our lab is studying the molecular mechanisms by which stress early in life modifies the development of circuits that regulate complex behaviors in the mouse. We are looking for a Postdoctoral Associate with expertise in neuroimmunology to help with a project examining the mechanisms by which alterations in microglial activity during a critical period of development contributes to some of the long-term behavioral changes seen in mice exposed to early life stress (Johnson and Kaffman 2017). The work will involve purifying microglia from the developing and adult mouse brain, harvesting RNA and DNA for genomic studies, immunohistochemistry and ISH studies, and viral administration to newborn pups and adult mice.
1.Extensive experience with harvesting microglia (or other immune cells) using Percoll gradient and FACS.
2. Experience with molecular biology and biochemistry that includes harvesting and quantifying RNA and DNA, making cDNA, q-PCR, protein purification/quantification and western blot.
3. Previous exposure to genomic studies is desirable.
4. Experience with tissue collection and processing with mice that includes dissection, perfusion, slicing tissue and IHC.
5. Solid background in neuroscience and neurodevelopment is highly desired.
6. Experience with confocal microscopy, intracranial stereotactic injection and conducting behavioral work in rodents are desirable.
7.Excellent verbal and writing skills in English are essential.