Postdoc Fellow - Single Neuron Transcriptomics and Regulation of Hunger
BIDMC and Harvard Medical School
June 21, 2018
NIH Postdoc payscale
Full Time - Experienced
Academic / Research
Topic: Single neuron transcriptomic analyses of brain regions regulating hunger, other homeostatic motivational drives, and physiology.
Laboratory: Bradford B. Lowell MD, PhD, Professor of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts. Lab website: https://www.lowelllab.com
The Postdoc Fellow will work with a highly collaborative team that has state-of-the-art expertise in single neuron and nuclei transcriptomics (Drop-seq, sNuc-seq, etc.), bioinformatic analysis of single cell datasets to determine a “parts list” for each brain site (see Campbell JN, et al., Nat Neurosci, 2017), rapid CRISPR/Cas9-mediated generation of recombinase driver mice to provide experimental access to newly discovered “parts” (i.e. neurons), and finally state-of-the-art expertise in systems neuroscience including: 1) analysis of neural circuitry in mice, 2) manipulation of neural activity, in vivo, using optical and/or chemogenetic approaches, 3) monitoring of neural activity in vivo with Ca2+-based imaging and/or optetrode technologies, 4) brain slice electrophysiology, and 5) advanced viral technologies for circuit mapping, manipulation of gene expression and/or for altering neuronal activity.
Recent publications from our collaborative research group:
Campbell JN, Macosko EZ, Fenselau H, Pers TH, Lyubetskaya A, Tenen D, Goldman M, Verstegen AM, Resch JM, McCarroll SA, Rosen ED**, Lowell BB**, Tsai LT**. A molecular census of arcuate hypothalamus and median eminence cell types. Nat Neurosci 20: 484-496, 2017.
Livneh Y, Ramesh RN, Burgess CR, Levandowski KM, Madara JC, Fenselau H, Goldy GJ, Diaz VE, Jikomes N, Resch JM, Lowell BB**, Andermann ML**. Homeostatic circuits selectively gate food cue responses in insular cortex. Nature 546: 611-616, 2017
Fenselau H, Campbell JN, Verstegen AMJ, Madara JC, Xu J, Shah BP, Resch JM, Yang Z, Mandelblat-Cerf Y, Livneh Y and Lowell BB. A rapidly-acting glutamatergic ARCàPVH satiety circuit postsynaptically regulated by a-MSH. Nat Neurosci 20: 42-51, 2017.
Resch JM, Fenselau H, Madara JC*, Wu C, Campbell JN, Lyubetskaya A, Dawes BA, Tsai LT, Li MM, Livneh Y, Ke Q, Kang PM, Fejes-Tóth G, Náray-Fejes-Tóth A, Geerling JC**, Lowell BB**. Aldosterone-sensing neurons in the NTS exhibit state-dependent pacemaker activity and drive sodium appetite via synergy with angiotensin II signaling. Neuron 96, 190–206, 2017.
Mandelblat-Cerf Y, Kim A, Subramanian S, Burgess CR, Tannous BA, Lowell BB**, Andermann ML**. Bidirectional anticipation of future osmotic challenges by vasopressin neurons. Neuron 93: 57-65, 2017.
Garfield AS**, Shah BP, Burgess CR, Li MM, Li C, Steger JS, Madara JC, Campbell JN, Kroeger D, Scammell TE, Tannous BA, Myers Jr MG, Andermann**, Krashes MJ**, Lowell BB**. Dynamic GABAergic afferent modulation of AgRP neurons. Nat Neurosci 19: 1628-1635, 2016.
Kong D, Dagon Y, Campbell JN, Guo Y, Yang Z, Yi X, Aryal P, Wellenstein K, Kahn BB**, Sabatini BL**, Lowell BB**. A postsynaptic AMPKàp21-activated kinase pathway drives fasting-induced synaptic plasticity in AgRP neurons. Neuron 91: 25-33, 2016.
Garfield AS, Li C, Madara JC, Shah BP, Webber E, Steger JS, Campbell JN, Gavrilova O, Lee CE, Olson DP, Elmquist JK, Tannous BA, Krashes MJ**, Lowell BB**. A neural basis for melanocortin-4 receptor regulated appetite. Nat Neurosci 18: 863-71, 2015.
Mandelblat-Cerf Y, Ramesh RN, Burgess CR, Patella P, Yang Z, Lowell BB, Andermann ML. AgRP and putative POMC neurons show opposite changes in spiking across multiple timescales. eLife. 2015;4.
Krashes MJ, Shah BP, Madara JC, Olson DP, Strochlic DE, Garfield AS, Vong L, Pei H, Watabe-Uchida M, Uchida N, Liberles SD, Lowell BB. An excitatory paraventricular nucleus to AgRP neuron circuit that drives hunger. Nature 507: 238-42, 2014.
Letters of application and CVs should be emailed to:
Bradford B. Lowell, MD, PhD - firstname.lastname@example.org
Professor of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School
Harvard Medical School and Beth Israel Deaconess Medical Center are Equal Opportunity/Affirmative Action Employers. Women and minorities are particularly encouraged to apply.
Candidates must have PhD, MD, or MD/PhD degrees. Ideal candidates will have experience with RNA-seq, facility with statistics and analyses of big data sets, and be interested in neural circuits controlling homeostatic motivational drives and physiology. Basic programming experience, though not strictly required, is preferred.