Postdoctoral Research Associate - Regulatory Biology Laboratory, Dr. Satchin Panda
The Salk Institute For Biological Studies
March 1, 2018
La Jolla, California
Full Time - Experienced
Academic / Research
We are currently seeking a postdoctoral fellow with a long term career interest in studying the neural basis of circadian rhythm and its entrainment to light-dark cycle. The neurobiological research aspect in the laboratory focuses on molecular and circuit mechanisms of circadian and diurnal rhythms, using a combination of behavioral, electrophysiology, trans-synaptic viral tracing, next-gen sequencing, serial blockface electron microscopy, advance fluorescent based imaging techniques. The successful candidate will employ electrophysiology, SBEM, advance imaging, together with engineered viruses to dissect the physical and functional connectivity underlying adaptation of animals to their ambient lighting condition.
We primarily study the intrinsically photosensitive retinal ganglion cells (ipRGCs) and their interaction with several target brain regions including the Suprachiasmatic Nucleus (SCN), olivary pretectal nucleus (OPN), lateral geniculate in the context of their respective circuitry. We have discovered that the ipRGCs are principal conduits of all light input to non-image forming visual responses in mammals. However, the molecular and circuit mechanisms underlying how mammals adapt to ambient lighting condition through ipRGC-based pathways is not understood. ipRGCs affect a range of brain functions including but not limited to photoentrainment of circadian rhythm, pupillary light reflex, brightness perception in visual scene, photophobia, migraine pain, depression, alertness, sleep, melatonin release, and possibly certain type of epilepsy. Therefore, understanding ipRGC function has broad impact in both basic science and translational research. We have generated several mouse lines and a series of engineered viruses to specifically label or perturb ipRGCs or their target cell types in the brain. In parallel, we have optimized multiscale imaging methods from light microscopy to large volume SBEM to map the neural circuits underlying ipRGC functions. The laboratory is well equipped with electrophysiology, animal behavior, and molecular biology tools for this project. The successful candidate will work with respective scientific cores of Salk Institute in areas of shared resources.
Education: The candidate should hold a doctoral degree with a strong background in neuroscience.
Experience: Experience on electrophysiological recording, animal behavior, SBEM, or advance imaging techniques is preferred, with computational background considered an advantageous. Training will be provided in all necessary areas for successful completion of the project.
Application Instructions: Applicants should send curriculum vitae, statement of research interests and experience, and three references to:
Satchidananda Panda, PhD Regulatory Biology Laboratory Salk Institute for Biological Studies 10010 North Torrey Pines Road La Jolla, CA 92037 Email: firstname.lastname@example.org
1. Brown, T.M., Gias, C., Hatori, M., Keding, S.R., Semo, M., Coffey, P.J., Gigg, J., Piggins, H.D., Panda, S., and Lucas, R.J. (2010). Melanopsin contributions to irradiance coding in the thalamo-cortical visual system. PLoS Biol 8, e1000558.
2. Hatori, M., Gill, S., Mure, L.S., Goulding, M., O'Leary, D.D., and Panda, S. (2014). Lhx1 maintains synchrony among circadian oscillator neurons of the SCN. eLife 3, e03357.
3. Hatori, M., Le, H., Vollmers, C., Keding, S.R., Tanaka, N., Schmedt, C., Jegla, T., and Panda, S. (2008). Inducible ablation of melanopsin-expressing retinal ganglion cells reveals their central role in non-image forming visual responses. PLoS ONE 3, e2451.
4. Hatori, M., and Panda, S. (2010). The emerging roles of melanopsin in behavioral adaptation to light. Trends Mol Med 16, 435-446.
5. Jones, K.A., Hatori, M., Mure, L.S., Bramley, J.R., Artymyshyn, R., Hong, S.P., Marzabadi, M., Zhong, H., Sprouse, J., Zhu, Q., et al. (2013). Small-molecule antagonists of melanopsin-mediated phototransduction. Nature chemical biology 9, 630-635.
6. Kofuji, P., Mure, L.S., Massman, L.J., Purrier, N., Panda, S., and Engeland, W.C. (2016). Intrinsically Photosensitive Retinal Ganglion Cells (ipRGCs) Are Necessary for Light Entrainment of Peripheral Clocks. PLoS ONE 11, e0168651.
7. Lin, B., Koizumi, A., Tanaka, N., Panda, S., and Masland, R.H. (2008). Restoration of visual function in retinal degeneration mice by ectopic expression of melanopsin. Proc Natl Acad Sci U S A 105, 16009-16014.
8. Mao, C.A., Li, H., Zhang, Z., Kiyama, T., Panda, S., Hattar, S., Ribelayga, C.P., Mills, S.L., and Wang, S.W. (2014). T-box Transcription Regulator Tbr2 Is Essential for the Formation and Maintenance of Opn4/Melanopsin-Expressing Intrinsically Photosensitive Retinal Ganglion Cells. J Neurosci 34, 13083-13095.
9. Mure, L.S., Hatori, M., Zhu, Q., Demas, J., Kim, I.M., Nayak, S.K., and Panda, S. (2016). Melanopsin-Encoded Response Properties of Intrinsically Photosensitive Retinal Ganglion Cells. Neuron.
10. Panda, S., Nayak, S.K., Campo, B., Walker, J.R., Hogenesch, J.B., and Jegla, T. (2005). Illumination of the melanopsin signaling pathway. Science 307, 600-604.
11. Panda, S., Provencio, I., Tu, D.C., Pires, S.S., Rollag, M.D., Castrucci, A.M., Pletcher, M.T., Sato, T.K., Wiltshire, T., Andahazy, M., et al. (2003). Melanopsin is required for non-image-forming photic responses in blind mice. Science 301, 525-527.
Internal Number: F894
About The Salk Institute For Biological Studies
The Salk Institute for Biological Studies is one of the world's preeminent basic research institutions. It consistently ranks among the top US institutions in terms of research output and quality in the life sciences. The institute provides a superb, collaborative working environment with a focus on mentoring future generations of young researchers.