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Scientist I - Stem Cells and Gene Editing

Allen Institute for Cell Science
Seattle, Washington State
Starting at $90,000 per year + benefits
Closing date
Nov 25, 2022
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The mission of the Allen Institute is to unlock the complexities of bioscience and advance our knowledge to improve human health. Using an open science, multi-scale, team-oriented approach, the Allen Institute focuses on accelerating foundational research, developing standards and models, and cultivating new ideas to make a broad, transformational impact on science.

The mission of the Allen Institute for Cell Science is to create multi-scale visual models of cell organization, dynamics, and activities. Our approach encompasses large scale data collection, observation, theory, and predictions to understand cellular behavior in normal and pathological contexts. As a division within the Allen Institute, the Allen Institute for Cell Science uses a team-oriented approach, focusing on accelerating foundational research, developing standards and models, and cultivating new ideas to make a transformational impact on science.

We seek a motivated, knowledgeable, and team-oriented scientist with excellent technical skills to join the Stem Cells and Gene Editing research team in the Allen Institute for Cell Science. The scientist will focus on genome engineering using CRISPR/Cas9 and related tools in human induced pluripotent stem cells (hiPSCs) for various internal and collaborative scientific projects.

Projects will include fluorescent protein fusion tagging at endogenous loci and genetic perturbations using CRISPRi and related technologies, introducing disease mutations, and performing quality control and experimental assays. The successful candidate will apply established protocols for gene editing and quality control to optimize and develop new methods and workflows, with the goal to collaboratively explore cell states and generate research publications.

The scientist will also assist with program/project management in collaboration with imaging-focused teams to achieve scientific deliverables. The successful candidate will have a strong background and expertise in molecular biology and cell culture, extensive knowledge of genome editing technologies and an interest in working closely with an interdisciplinary team to achieve the institute’s scientific goals. The successful candidate will also have an interest in developing a range of invaluable tools for cell biology research both within and outside of the institute.

The Allen Institute believes that team science significantly benefits from the participation of diverse voices, experiences and backgrounds. High-quality science can only be produced when it includes different perspectives. We are committed to increasing diversity across every team and encourage people from all backgrounds to apply for this role.

Essential Functions

  • Generate and maintain multiple gene-edited human iPSC lines
  • Routinely perform molecular biology experiments, including PCR and PCR-based assays, including ddPCR, molecular cloning and sequencing based assessment of genome editing outcomes
  • Help develop and optimize methods for gene editing and stem cell quality control, with experiments and collaboratively through project meetings and at conferences and for publications
  • Help plan and execute experiments focused on perturbation with CRISPR/Cas9 and related technologies
  • Help plan and execute experiments focused on image-based quantitative assays of cell behavior
  • Help the team remain current on cutting edge literature and help manage external collaborations
  • Adhere to SOPs and workflows with meticulous documentation
  • Properly handle hazardous chemicals
  • Assist cross-functional activities including hand-offs to various teams, compiling data, and presenting

Note: Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions. This description reflects management’s assignment of essential functions; it does not proscribe or restrict the tasks that may be assigned.

Required Education and Experience

  • PhD in molecular, cell biology, or a related biological life science or equivalent combination of degree and experience

Preferred Education and Experience

  • Experience in molecular biology
  • Experience with Cas9/CRISPR and/or related genome editing methods
  • Experience with mammalian cell culture with a preference for stem cells
  • Experience with virus packaging and delivery
  • Ability to follow instructions and protocols precisely and reliably
  • Excellent written and verbal communication skills
  • Excellent interpersonal skills and ability to collaborate within a team-based environment
  • Strong organizational skills and careful attention to detail

Work Environment:

  • May enter laboratory environment, including potential exposure to lasers, biohazards

Physical Demands:

  • Occasional lifting to 30 pounds
  • Fine motor movements in fingers/hands to operate computers and other office equipment; lab equipment

Position Type/Expected Hours of Work:

  • This role is currently working onsite and remotely when possible, due to COVID-19 and our focus on employee safety. We continue to evaluate the safest options for our employees.

Additional Eligibility Qualifications

  • In keeping with our focus on employee safety, all employees must be fully vaccinated against COVID-19 as a condition of employment unless a medical or religious exemption is approved. All employees will be required to keep their vaccination status up to date according to CDC guidance and our policy.

Additional Comments

  • **Please note, this opportunity offers relocation assistance and work visa sponsorship**

It is the policy of the Allen Institute to provide equal employment opportunity (EEO) to all persons regardless of age, color, national origin, citizenship status, physical or mental disability, race, religion, creed, gender, sex, sexual orientation, gender identity and/or expression, genetic information, marital status, status with regard to public assistance, veteran status, or any other characteristic protected by federal, state or local law. In addition, the Allen Institute will provide reasonable accommodations for qualified individuals with disabilities.

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