Postdoctoral position at the NIH in motor protein function in cerebellar Purkinje neurons
A fully-funded postdoctoral position is available for a highly-motivated scientist who would like to use genetic, cell biological, and advanced imaging approaches to explore the functions of motor proteins in cerebellar Purkinje neurons, the master neuron of the cerebellum. We have developed techniques to culture and transfect these neurons (J. Neurosci. Meth. 2011), perform miRNA-mediated protein knockdown in them (Meth. Cell Biol. 2016), identify novel protein: protein interactions in them (Cytoskeleton, 2018), employ complementation to define structure-function relationships in them (Nat. Cell Biol. 2011 and unpublished), and generate them from stem cells (Cerebellum 2019). Candidate motor proteins for investigation where we have already made knockout mice include myosin 10 (implicated in adhesion molecule targeting), myosin 18A (implicated in spine maturation; FASEB J, 2021) and myosin 19 (implicated in mitochondrial motility and positioning). The lab and institute offer a fantastic, highly-collaborative training environment for budding young scientists, with cutting-edge resources, microscopes, and expertise, as well as rigorous mentoring and generous support for trainees.