Research Assistant II

At Boston Children’s Hospital, the quality of our care – and our inclusive hospital working environment – lies in the diversity of our people. With patients from local communities and 160 countries around the world, we’re committed to reflecting the spectrum of their cultures, while opening doors of opportunity for our team. Here, different talents pursue common goals. Voices are heard and ideas are shared. Join us, and discover how your unique contributions can change lives. Yours included.

Project Description:
The Research Assistant  will be involved in the generation of knockout zebrafish lines and will work alongside a postdoctoral research fellow to characterize this zebrafish larvae using a variety of techniques including PCR, qPCR, western blotting, CRISPR-mediated gene editing, RNA sequencing, immunohistochemistry, microscopy and automated analyses of motor behavior.
The Research Assistant will maintain genetically-modified zebrafish lines. Methods include the use of CRISPR-Cas9, stereotactic oocyte injections, genotyping and zebrafish husbandry. Basic design of experiments and statistics will be taught or current skills enhanced. 

This Research Assistant will also be responsible for:

• According to prescribed protocols and assigned schedules, performs a variety of routine laboratory experiments and procedures, utilizing standard laboratory equipment, recording and checking results for completeness and accuracy.
• Assists in troubleshooting procedures if experimental results are unexpected or quality of specimens is poor.
• Maintains records of results and methodologies in a log of work performed.
• Maintains equipment, lab area and supplies in a neat professional manner.
• Sterilize and clean glassware, prepare media and other materials using appropriate procedures.
• Inventories supplies alerts supervisor to needed supplies, or mixes standard reagents.
• Processes order for supplies and/or animals. Attends courses in safety procedures for handling, storage and disposal of biohazardous, flammable, corrosive and toxic materials.
• Carries out all laboratory activities in compliance with applicable regulations and protocols.
• Conducts literature searches and reviews, compiling information for journals and suggesting uses for new techniques to the P.I.
• Attends courses, lectures and conferences to further own knowledge of research and laboratory techniques, consulting with other researchers and technicians to exchange information.
• Contributes to the team within and between departments and organizations.

To qualify, you must have:

• The knowledge of theories, principles, and concepts acquired through completion of a Bachelor of Science degree and one year of post grad relatable experience. 
• Analytical skills to resolve problems that require the use of basic scientific, mathematical, or technical principles.
• Ability to communicate effectively both orally and in writing
• Prior experience with common molecular biology techniques and/or handling zebrafish is preferred but not required

Please note: During a public health emergency, individuals in this role may be expected to take on additional duties to respond to organizational needs.

Boston Children’s Hospital offers competitive compensation and unmatched benefits, including a rotating days/evenings and week-end schedule, affordable health, vision and dental insurance, generous levels of time off, 403(b) Retirement Savings plan, Pension, Tuition Reimbursement, cell phone plan discounts and discounted rates on T-passes (50% off). Discover your best.