Location: Paris Descartes Université, Inserm U1128, Paris, France
Start date: early 2018
Several postdoc positions available:
Our team aims at understanding how interactions between neurons and non-neuronal cells modulate brain activity and how this modulation contributes to the signals used in functional imaging of the human brain. This imposes to work at all spatial and temporal scales and to understand deeply the relationship between the activity of a given neuronal network and the nature of the imaging signal used to detect it.
Using cellular approaches (electrophysiology, two-photon fluorescence and phosphorescence microscopy), we investigate the functional responses of the glomerulus network, i.e. the responses to odor of local neurons, glial cells and vascular cells (pericytes and smooth muscle cells), and we correlate these responses with metabolic (oxygen, phosphorescence lifetime imaging) and macroscopic vascular responses measured with fast ultrasound imaging and BOLD MRI.
The candidates should have a background in synaptic and cellular electrophysiology, in in vitro or in vivo imaging, or in optics.
The projects are financed by several long term grants (ERC Advanced Grant, Leducq Foundation, NSF/ANR and RHU). The positions have 2 years of initial funding, with the possibility for extension.
Candidates should apply through the career center or send a CV and a brief statement of research experience to Serge Charpak:
Selection of publications from the host team (2013-2017):
Rungta et al. Nat. Commun. (2017)
G. Lyons et al, Elife. (2016).
Otsu et al., Nat. Neurosci. (2015).
Najac et al., J Neurosci (2015).
Parpaleix, Nat Medicine (2013).
Ducros et al., PNAS (2013).
Background in synaptic and cellular electrophysiology, in in vitro or in vivo imaging, or in optics.
Additional Salary Information: According to the career and INSERM regulations
Charpak’s initial interests focused on how synaptic transmission and intrinsic membrane properties of neurons contribute to information processing in the central nervous system. In 1998, he started to use two-photon microscopy to image neuronal activity in vivo and more recently neurovascular coupling and brain metabolism. Selected old papers:-Charpak S, Gahwiler BH, Do KQ, Knopfel T (1990) Potass...ium conductances in hippocampal neurons blocked by excitatory amino-acid transmitters. Nature 347: 765-767. -Charpak S, Gahwiler BH (1991) Glutamate mediates a slow synaptic response in hippocampal slice cultures. Proc R Soc Lond B Biol Sci 243: 221-226 -Scanziani M, Gahwiler BH, Charpak S (1998) Target cell-specific modulation of transmitter release at terminals from a single axon. Proc Natl Acad Sci U S A 95: 12004-12009. -Charpak S, Mertz J, Beaurepaire E, Moreaux L, Delaney K (2001) Odor-evoked calcium signals in dendrites of rat mitral cells. Proc Natl Acad Sci U S A 98: 1230-1234. -Debarbieux F, Audinat E, Charpak S (2003) Action potential propagation in dendrites of rat mitral cells in vivo. J Neurosci 23: 5553-5560. -Oheim M, Beaurepaire E, Chaigneau E, Mertz J, Charpak S (2001) Two-photon microscopy in brain tissue: parameters influencing the imaging depth. J Neurosci Methods 111: 29-37. -Chaigneau E, Oheim M, Audinat E, Charpak S (2003) Two-photon imaging of capillary blood flow in olfactory bulb glomeruli. Proc Natl Acad Sci U S A 100: 13081-13086. -Chaigneau E, Tiret P, Lecoq J, Ducros M, Knopfel T, Charpak S (2007) The relationship between blood flow and neuronal activity in the rodent olfactory bulb. J Neurosci 27:6452-6460. -Lecoq, J, Parpaleix, A, Roussakis, E, Ducros, M., Goulam, Y, Vinogradov, S, Charpak, S (2011) Simultaneous two-photon imaging of oxygen and bloodflow in deep cerebral vessels. Nature Med 17: 893-898.