A post doctoral position in the Department of Anesthesiology at the University of Illinois in Chicago, to work on studies funded by the National Multiple Sclerosis Society (NMSS). The main goal is to understand mechanisms by which LKE (lanthionine ketimine ester) provides benefit in the EAE mouse model of MS mediated through interactions with its protein target CRMP2 (Dupree et al, 2015, J Neurochem. 134:302). EAE studies will be carried out in mice with conditional neuronal knock out of CRMP2 and of CRMP2 phosphorylation. Studies will determine effects of CRMP2 on clinical scores, neuropathology, and response to LKE. Studies will include RNAseq, Ribotag, and use of Thy1-GFP mice to visualize injury. In vitro studies with primary neurons and oligodendrocytes will explore role of CRMP2 in neuronal and oligodendrocyte maturation.
Experience with one or more of the following: EAE; characterization of neuropathology; preparation of primary oligodendrocytes; analysis of protein phosphorylation.
Additional Salary Information: Will follow NIH guidelines; renewable dependent upon performance and funding availability