The specific duties of the lab technician will be to use whole-cell patching methods to probe the cellular diversity of brain circuits across cortical areas and species at the level of morphology, electrophysiology and transcriptome.
The laboratory of Dr. Andreas Tolias in the Department of Neuroscience at Baylor College of Medicine is seeking applicants for lab technician research positions. The lab technicians will work collaboratively within a multi-disciplinary research team aimed at understanding the cellular diversity of brain circuits. The specific goals of this position will be to use whole-cell patching methods, single-cell RNA-sequencing and immunochemistry to probe morphology, electrophysiology and transcriptome of the individual neurons of brain circuits across cortical areas and species. In particular, Patch-seq, a method we recently developed, that combines whole-cell patch-clamp recordings and single-cell RNA sequencing (scRNA-seq). The lab tech will receive extensive one-on-one training with postdocs on cellular electrophysiology to develop the necessary skills to acquire high-quality data.
The ideal applicant should have some experience in cellular electrophysiology or RNA-sequencing. The candidates without these experiences will be also considered if they are willing to commit the efforts to develop these skills.
You can learn more about our research at http://toliaslab.org and more specific information about the current project in recent publications from our lab:
Jiang X, Shen S, Cadwell CR, Berens P, Sinz F, Ecker AS, Patel S & Tolias AS (2015). Principles of Connectivity among Morphologically Defined Cell Types in Adult Neocortex. Science. Vol. 350 no. 6264 DOI: 10.1126
Cadwell CR, Palasantza A, Jiang X, Berens P, Deng Q, Yilmaz M, Reimer J, Shen S, Bethge M, Tolias KF, Sandberg R & Tolias AS (2015). Morphological, electrophysiological and transcriptomic profiling of single neurons using Patch-seq. Nature Biotechnology
Required: Bachelor's degree in a basic science or equivalent education.
Preferred: Master's degree in a basic science.
One year of directly related experience is preferred.